rabbit anti human rab7 (Cell Signaling Technology Inc)
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Rabbit Anti Human Rab7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 582 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+human+rab7/Rab7+Antibody/pm38877304-326-145-150
Average 96 stars, based on 582 article reviews
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Immunofluorescence:Article Title: Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Article Snippet: .. Antibodies for immunofluorescence were mouse IgG2b anti-human CD63 (clone TS63b35 1/100, available upon request to E. Rubinstein: eric.rubinstein@inserm.fr) and mouse IgG1 anti-human CD9 (clone TS936 1/100) (commercially available at Diaclone or Abcam), mouse IgG1 antihuman EEA1 (BD Transduction Laboratories, clone 14/EEA1, 1/1000), mouse IgG2a anti-human RAB5 (BD Transduction Laboratories, clone 1/Rab5, 1/100), Article Title: Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 Article Snippet: Antibodies for immunoprecipitation were mouse anti-human CD63 (BD Bioscience, clone H5C6, 1/250), mouse anti-human CD9 (Millipore, clone MM2/57, 1/50), normal mouse IgG (Millipore, 1/500); a rabbit anti-GFP polyclonal antibody was produced for the TAb-IP antibody facility and purified by the recombinant protein facility of Institut Curie (for exclusive use by Institut Curie researchers) and used at 1.5 μg/mL. .. Antibodies for immunofluorescence were mouse IgG2b anti-human CD63 (clone TS63b 1/100, available upon request to E. Rubinstein: eric.rubinstein@inserm.fr) and mouse IgG1 anti-human CD9 (clone TS9 1/100) (commercially available at Diaclone or Abcam), mouse IgG1 anti-human EEA1 (BD Transduction Laboratories, clone 14/EEA1, 1/1000), mouse IgG2a anti-human RAB5 (BD Transduction Laboratories, clone 1/Rab5, 1/100), Blocking Assay:Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions. Article Snippet: Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices.. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions.. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood. Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions Article Snippet: .. The fixed and PBS-washed samples were permeabilized with 0.15% Triton x-100 in PBS for 15′, washed three times with PBS and blocked with 3% bovine serum albumin (BSA) in PBS for 1 h. Primary antibodies were diluted in blocking buffer supplemented with 0.1% Triton x-100 and the samples were incubated overnight at 4 °C with the following antibodies: Purified mouse anti-human VE-cadherin (BD bioscience, 555661; dilution of 1:200) (Figs. A, I, and ; Appendix ); rabbit anti-human VE-cadherin (D87F2, Cell signaling, 2500S; 1:200) (Figs. A, and , G, C, ; Appendix ); rabbit anti-human RAB6 (D37C7, Cell signaling, 9625S; 1:100) (Figs. H,I, I, and Appendix Fig. ); Incubation:Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions. Article Snippet: Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices.. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions.. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood. Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions Article Snippet: .. The fixed and PBS-washed samples were permeabilized with 0.15% Triton x-100 in PBS for 15′, washed three times with PBS and blocked with 3% bovine serum albumin (BSA) in PBS for 1 h. Primary antibodies were diluted in blocking buffer supplemented with 0.1% Triton x-100 and the samples were incubated overnight at 4 °C with the following antibodies: Purified mouse anti-human VE-cadherin (BD bioscience, 555661; dilution of 1:200) (Figs. A, I, and ; Appendix ); rabbit anti-human VE-cadherin (D87F2, Cell signaling, 2500S; 1:200) (Figs. A, and , G, C, ; Appendix ); rabbit anti-human RAB6 (D37C7, Cell signaling, 9625S; 1:100) (Figs. H,I, I, and Appendix Fig. ); Purification:Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions. Article Snippet: Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices.. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions.. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood. Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions Article Snippet: .. The fixed and PBS-washed samples were permeabilized with 0.15% Triton x-100 in PBS for 15′, washed three times with PBS and blocked with 3% bovine serum albumin (BSA) in PBS for 1 h. Primary antibodies were diluted in blocking buffer supplemented with 0.1% Triton x-100 and the samples were incubated overnight at 4 °C with the following antibodies: Purified mouse anti-human VE-cadherin (BD bioscience, 555661; dilution of 1:200) (Figs. A, I, and ; Appendix ); rabbit anti-human VE-cadherin (D87F2, Cell signaling, 2500S; 1:200) (Figs. A, and , G, C, ; Appendix ); rabbit anti-human RAB6 (D37C7, Cell signaling, 9625S; 1:100) (Figs. H,I, I, and Appendix Fig. ); other:Article Title: Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. Article Snippet: Control mice received equal amounts of isotope control antibodies (Cat# BE0091, BioXcell), along with 4D5 or IgG, as previously described (Coffelt et al., 2015). Article Title: Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. Article Snippet: In some experiment, ErbB2 Tgmice were treated with 50 mg twice weekly anti-IL-1b (Cat# BE0246, BioXCell) in the presence of 4D5 or IgG. |

