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rabbit anti human rab7  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti human rab7
    Rabbit Anti Human Rab7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 582 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+rab7/Rab7+Antibody/pm38877304-326-145-150
    Average 96 stars, based on 582 article reviews
    rabbit anti human rab7 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9.
    Article Snippet: .. Antibodies for immunofluorescence were mouse IgG2b anti-human CD63 (clone TS63b35 1/100, available upon request to E. Rubinstein: eric.rubinstein@inserm.fr) and mouse IgG1 anti-human CD9 (clone TS936 1/100) (commercially available at Diaclone or Abcam), mouse IgG1 antihuman EEA1 (BD Transduction Laboratories, clone 14/EEA1, 1/1000), mouse IgG2a anti-human RAB5 (BD Transduction Laboratories, clone 1/Rab5, 1/100), rabbit anti-human RAB7 (Cell Signaling, D95F2, 1/100), mouse IgG1 anti-human LAMP1 (Developmental Studies Hybridoma Bank, H4A3, 1/400), goat anti-mouse IgG2b Alexafluor 647 (Invitrogen, 1/300), goat anti-mouse IgG1 Alexafluor 488 (Invitrogen 1/300), goat anti-mouse IgG Alexafluor 568 (Invitrogen, 1/200), goat anti-rabbit IgG Alexafluor 568 (Invitrogen, 1/200), goat anti-rabbit IgG Alexafluor 647 (Invitrogen, 1/200). ..

    Article Title: Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
    Article Snippet: Antibodies for immunoprecipitation were mouse anti-human CD63 (BD Bioscience, clone H5C6, 1/250), mouse anti-human CD9 (Millipore, clone MM2/57, 1/50), normal mouse IgG (Millipore, 1/500); a rabbit anti-GFP polyclonal antibody was produced for the TAb-IP antibody facility and purified by the recombinant protein facility of Institut Curie (for exclusive use by Institut Curie researchers) and used at 1.5 μg/mL. .. Antibodies for immunofluorescence were mouse IgG2b anti-human CD63 (clone TS63b 1/100, available upon request to E. Rubinstein: eric.rubinstein@inserm.fr) and mouse IgG1 anti-human CD9 (clone TS9 1/100) (commercially available at Diaclone or Abcam), mouse IgG1 anti-human EEA1 (BD Transduction Laboratories, clone 14/EEA1, 1/1000), mouse IgG2a anti-human RAB5 (BD Transduction Laboratories, clone 1/Rab5, 1/100), rabbit anti-human RAB7 (Cell Signaling, D95F2, 1/100), mouse IgG1 anti-human LAMP1 (Developmental Studies Hybridoma Bank, H4A3, 1/400), goat anti-mouse IgG2b Alexafluor 647 (Invitrogen, 1/300), goat anti-mouse IgG1 Alexafluor 488 (Invitrogen 1/300), goat anti-mouse IgG Alexafluor 568 (Invitrogen, 1/200), goat anti-rabbit IgG Alexafluor 568 (Invitrogen, 1/200), goat anti-rabbit IgG Alexafluor 647 (Invitrogen, 1/200). .. Mouse anti-GFP-Alexa fluor 647 (BD Biosciences, clone 1A12-6-18, 1/150) was purchased.

    Blocking Assay:

    Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions.
    Article Snippet: Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices.. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions.. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood.

    Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions
    Article Snippet: .. The fixed and PBS-washed samples were permeabilized with 0.15% Triton x-100 in PBS for 15′, washed three times with PBS and blocked with 3% bovine serum albumin (BSA) in PBS for 1 h. Primary antibodies were diluted in blocking buffer supplemented with 0.1% Triton x-100 and the samples were incubated overnight at 4 °C with the following antibodies: Purified mouse anti-human VE-cadherin (BD bioscience, 555661; dilution of 1:200) (Figs. A, I, and ; Appendix ); rabbit anti-human VE-cadherin (D87F2, Cell signaling, 2500S; 1:200) (Figs. A, and , G, C, ; Appendix ); rabbit anti-human RAB6 (D37C7, Cell signaling, 9625S; 1:100) (Figs. H,I, I, and Appendix Fig. ); rabbit anti-human RAB7 (D95F2 XP, Cell signaling 9367TS; 1:200) (Fig. ); mouse anti-human CCL2 (24822, R&D Systems, MAB279; 1:50) (Figs. G,I, G, and ; Appendix Fig. ); mouse anti-human α-Tubulin (Santa-Cruz biotechnology, sc-32293; 1:200) (Fig. ); mouse anti-human ELKS (E-1, Santa-Cruz biotechnology, sc-365715; 1:200) (Figs. and , ). .. After incubation with the primary antibodies, the cells were washed three times with PBS and fluorophore conjugated secondary antibodies (diluted 1:400 in blocking buffer), were added to the cells for 2 h. The following secondary antibodies were used: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, A21206 ); Alexa Fluor 488 donkey anti-mouse (Invitrogen, A21202 ); Alexa Fluor 594 donkey anti-mouse (Invitrogen, A21203 ); Alexa Fluor 647 donkey anti-rabbit (Invitrogen, A31573 ); Alexa Fluor 647 donkey anti-mouse (Invitrogen, A31571 ).

    Incubation:

    Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions.
    Article Snippet: Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices.. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions.. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood.

    Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions
    Article Snippet: .. The fixed and PBS-washed samples were permeabilized with 0.15% Triton x-100 in PBS for 15′, washed three times with PBS and blocked with 3% bovine serum albumin (BSA) in PBS for 1 h. Primary antibodies were diluted in blocking buffer supplemented with 0.1% Triton x-100 and the samples were incubated overnight at 4 °C with the following antibodies: Purified mouse anti-human VE-cadherin (BD bioscience, 555661; dilution of 1:200) (Figs. A, I, and ; Appendix ); rabbit anti-human VE-cadherin (D87F2, Cell signaling, 2500S; 1:200) (Figs. A, and , G, C, ; Appendix ); rabbit anti-human RAB6 (D37C7, Cell signaling, 9625S; 1:100) (Figs. H,I, I, and Appendix Fig. ); rabbit anti-human RAB7 (D95F2 XP, Cell signaling 9367TS; 1:200) (Fig. ); mouse anti-human CCL2 (24822, R&D Systems, MAB279; 1:50) (Figs. G,I, G, and ; Appendix Fig. ); mouse anti-human α-Tubulin (Santa-Cruz biotechnology, sc-32293; 1:200) (Fig. ); mouse anti-human ELKS (E-1, Santa-Cruz biotechnology, sc-365715; 1:200) (Figs. and , ). .. After incubation with the primary antibodies, the cells were washed three times with PBS and fluorophore conjugated secondary antibodies (diluted 1:400 in blocking buffer), were added to the cells for 2 h. The following secondary antibodies were used: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, A21206 ); Alexa Fluor 488 donkey anti-mouse (Invitrogen, A21202 ); Alexa Fluor 594 donkey anti-mouse (Invitrogen, A21203 ); Alexa Fluor 647 donkey anti-rabbit (Invitrogen, A31573 ); Alexa Fluor 647 donkey anti-mouse (Invitrogen, A31571 ).

    Purification:

    Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions.
    Article Snippet: Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices.. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions.. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood.

    Article Title: Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions
    Article Snippet: .. The fixed and PBS-washed samples were permeabilized with 0.15% Triton x-100 in PBS for 15′, washed three times with PBS and blocked with 3% bovine serum albumin (BSA) in PBS for 1 h. Primary antibodies were diluted in blocking buffer supplemented with 0.1% Triton x-100 and the samples were incubated overnight at 4 °C with the following antibodies: Purified mouse anti-human VE-cadherin (BD bioscience, 555661; dilution of 1:200) (Figs. A, I, and ; Appendix ); rabbit anti-human VE-cadherin (D87F2, Cell signaling, 2500S; 1:200) (Figs. A, and , G, C, ; Appendix ); rabbit anti-human RAB6 (D37C7, Cell signaling, 9625S; 1:100) (Figs. H,I, I, and Appendix Fig. ); rabbit anti-human RAB7 (D95F2 XP, Cell signaling 9367TS; 1:200) (Fig. ); mouse anti-human CCL2 (24822, R&D Systems, MAB279; 1:50) (Figs. G,I, G, and ; Appendix Fig. ); mouse anti-human α-Tubulin (Santa-Cruz biotechnology, sc-32293; 1:200) (Fig. ); mouse anti-human ELKS (E-1, Santa-Cruz biotechnology, sc-365715; 1:200) (Figs. and , ). .. After incubation with the primary antibodies, the cells were washed three times with PBS and fluorophore conjugated secondary antibodies (diluted 1:400 in blocking buffer), were added to the cells for 2 h. The following secondary antibodies were used: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, A21206 ); Alexa Fluor 488 donkey anti-mouse (Invitrogen, A21202 ); Alexa Fluor 594 donkey anti-mouse (Invitrogen, A21203 ); Alexa Fluor 647 donkey anti-rabbit (Invitrogen, A31573 ); Alexa Fluor 647 donkey anti-mouse (Invitrogen, A31571 ).

    other:

    Article Title: Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.
    Article Snippet: Control mice received equal amounts of isotope control antibodies (Cat# BE0091, BioXcell), along with 4D5 or IgG, as previously described (Coffelt et al., 2015).

    Article Title: Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.
    Article Snippet: In some experiment, ErbB2 Tgmice were treated with 50 mg twice weekly anti-IL-1b (Cat# BE0246, BioXCell) in the presence of 4D5 or IgG.



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    Santa Cruz Biotechnology rabbit anti human rab7
    CRISPR LNPs Cell Trafficking. Rhodamine conjugated DHPE (1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine) phosphoglycerol tracked the locale of CRISPR LNPs in human MDMs. Confocal microscopy was employed 12 h after LNPs injection in the MDM cultures. Alexa-Fluor 488 (green) secondary antibody detected Rab 5, <t>Rab7,</t> or Lamp1 subcellular compartments. Phalloidin-iFluor 647 marked cell boundaries. The MDM nucleus was stained with DAPI (blue). Rhodamine DHPE phospholipid containing CRISPR-LNPs (red) colocalized with Rab5 (a) and Rab7 (green) (b). (c) No colocalization was found between Lamp1 (green) and the nanoparticles (red). (d) TM-Rhodamine labeled px333DE was used for CRISPR LNPs to examine nuclear localization of the CRISPR payload present in the nucleus 12h after treatment. Z-stack affirmed that the CRISPR reached the nucleus.
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    Cell Signaling Technology Inc anti human rab7
    Huh7-NTCP cells were grown on glass cover slides and fixed 48 hr after seeding. ( A ) Endogenous ERp57 with Rab5, <t>Rab7,</t> Rab11, or Lamp1 were immune-stained, and the colocalization of ERp57 (red channels) with Rab5, Rab7, Rab11, or Lamp1 (green channels) was analyzed by confocal microscopy. Scale bars of panels and zooms from squared area represent 10 µm and 2 µm, respectively. ( B ) The degree of colocalization between ERp57 and the different cell markers was assessed by determining the Pearson’s correlation coefficients with the JACoP plugin of ImageJ. Results are expressed as the mean of six individual cells. Error bars correspond to standard deviations.
    Anti Human Rab7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CRISPR LNPs Cell Trafficking. Rhodamine conjugated DHPE (1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine) phosphoglycerol tracked the locale of CRISPR LNPs in human MDMs. Confocal microscopy was employed 12 h after LNPs injection in the MDM cultures. Alexa-Fluor 488 (green) secondary antibody detected Rab 5, Rab7, or Lamp1 subcellular compartments. Phalloidin-iFluor 647 marked cell boundaries. The MDM nucleus was stained with DAPI (blue). Rhodamine DHPE phospholipid containing CRISPR-LNPs (red) colocalized with Rab5 (a) and Rab7 (green) (b). (c) No colocalization was found between Lamp1 (green) and the nanoparticles (red). (d) TM-Rhodamine labeled px333DE was used for CRISPR LNPs to examine nuclear localization of the CRISPR payload present in the nucleus 12h after treatment. Z-stack affirmed that the CRISPR reached the nucleus.

    Journal: EBioMedicine

    Article Title: CRISPR-Cas9 Mediated Exonic Disruption for HIV-1 Elimination

    doi: 10.1016/j.ebiom.2021.103678

    Figure Lengend Snippet: CRISPR LNPs Cell Trafficking. Rhodamine conjugated DHPE (1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine) phosphoglycerol tracked the locale of CRISPR LNPs in human MDMs. Confocal microscopy was employed 12 h after LNPs injection in the MDM cultures. Alexa-Fluor 488 (green) secondary antibody detected Rab 5, Rab7, or Lamp1 subcellular compartments. Phalloidin-iFluor 647 marked cell boundaries. The MDM nucleus was stained with DAPI (blue). Rhodamine DHPE phospholipid containing CRISPR-LNPs (red) colocalized with Rab5 (a) and Rab7 (green) (b). (c) No colocalization was found between Lamp1 (green) and the nanoparticles (red). (d) TM-Rhodamine labeled px333DE was used for CRISPR LNPs to examine nuclear localization of the CRISPR payload present in the nucleus 12h after treatment. Z-stack affirmed that the CRISPR reached the nucleus.

    Article Snippet: The following primary antibodies were used to label different endosomal compartments: Rabbit anti-human Rab5, (Abcam #ab18211), early endosomal marker, rabbit anti-human Rab7 (Santacruz biotechnology #sc10767), a late endosome marker, and rabbit anti-human Lamp-1 (lysosomal-associated membrane protein-1) (Abcam #ab24170) for lysosomes.

    Techniques: CRISPR, Confocal Microscopy, Injection, Staining, Labeling

    Huh7-NTCP cells were grown on glass cover slides and fixed 48 hr after seeding. ( A ) Endogenous ERp57 with Rab5, Rab7, Rab11, or Lamp1 were immune-stained, and the colocalization of ERp57 (red channels) with Rab5, Rab7, Rab11, or Lamp1 (green channels) was analyzed by confocal microscopy. Scale bars of panels and zooms from squared area represent 10 µm and 2 µm, respectively. ( B ) The degree of colocalization between ERp57 and the different cell markers was assessed by determining the Pearson’s correlation coefficients with the JACoP plugin of ImageJ. Results are expressed as the mean of six individual cells. Error bars correspond to standard deviations.

    Journal: eLife

    Article Title: A fusion peptide in preS1 and the human protein disulfide isomerase ERp57 are involved in hepatitis B virus membrane fusion process

    doi: 10.7554/eLife.64507

    Figure Lengend Snippet: Huh7-NTCP cells were grown on glass cover slides and fixed 48 hr after seeding. ( A ) Endogenous ERp57 with Rab5, Rab7, Rab11, or Lamp1 were immune-stained, and the colocalization of ERp57 (red channels) with Rab5, Rab7, Rab11, or Lamp1 (green channels) was analyzed by confocal microscopy. Scale bars of panels and zooms from squared area represent 10 µm and 2 µm, respectively. ( B ) The degree of colocalization between ERp57 and the different cell markers was assessed by determining the Pearson’s correlation coefficients with the JACoP plugin of ImageJ. Results are expressed as the mean of six individual cells. Error bars correspond to standard deviations.

    Article Snippet: Antibody , Anti-human Rab7 (rabbit monoclonal) , Cell Signaling Technology , (D95F2):9367 , IF (1:100).

    Techniques: Staining, Confocal Microscopy

    Journal: eLife

    Article Title: A fusion peptide in preS1 and the human protein disulfide isomerase ERp57 are involved in hepatitis B virus membrane fusion process

    doi: 10.7554/eLife.64507

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-human Rab7 (rabbit monoclonal) , Cell Signaling Technology , (D95F2):9367 , IF (1:100).

    Techniques: Virus, Produced, Variant Assay, Construct, Generated, Transduction, Retroviral, Plasmid Preparation, Selection, shRNA, Transfection, Expressing, Clone Assay, Luciferase, Isolation, Sequencing, Mutagenesis, Reporter Assay, Activity Assay, cDNA Synthesis, SYBR Green Assay, Membrane, Integrity Assay, Cytotoxicity Assay, Software, Staining, RNA Extraction